Cambridge Healthtech Institute Training Seminars offer real-life case studies, problems encountered and solutions applied, along with extensive coverage of the academic theory and background. Each Training Seminar offers a mix of formal lecture and interactive discussions and activities to maximize the learning experience. These Training Seminars are led by experienced instructors who will focus on content applicable to your current research and provide important guidance for those new to their fields.
TS9B: Antibody Deep Sequencing and Single Cell Analysis
WEDNESDAY, MAY 6 - THURSDAY, MAY 7
DAY 1: WEDNESDAY
8:40 am - 6:00 pm Seminar Sessions
12:30 - 1:30 Lunch Provided
6:00 - 7:15 Taste of New England Networking Reception in the Exhibit Hall with Poster Viewing
DAY 2: THURSDAY
8:30 am - 12:30 pm Seminar Sessions
Exhibit Hall Refreshment Breaks also provided.
Instructor:
Brandon DeKosky, PhD, Assistant Professor, Department of Chemical Engineering, Department of Pharmaceutical Chemistry, Kansas Vaccine Institute, The University of Kansas
Matías Gutiérrez González, PhD, Postdoctoral Researcher, Pharmaceutical Chemistry, The University of Kansas
In this training seminar, participants will learn about recently developed methods for Next-Generation Sequencing (NGS) and single-cell analysis of antibody repertoires. Part 1 will provide an introduction to antibody repertoires, including genetic
background, generation of diversity, and sequencing technologies. Part 2 will incorporate an introduction and hand-on session on computational tools for analyzing antibody repertoire NGS data. We will focus on pre-processing, analysis, and visualization
of data, along with presentation of existing bioinformatics pipelines available. Part 3 will focus on an overview of the development of newer methods in single-cell analysis of antibody immune responses. The course will be interactive with case
studies, participants will be able to download data and examples. Please bring your computer.
Topics to Be Discussed:
PART I: Introduction to NGS of Antibody Repertoires and Bioinformatics Tools
- General Introduction to Antibody Repertoire Analysis
- Experimental design and considerations
- Data analysis overview
- Hands-on session: Introduction of common computational tools for NGS
PART II: Preprocessing and Analysis of Antibody Repertoire NGS Data
- Hands-on session: Preprocessing data
- Hands-on session: Antibody immune repertoire analysis
- Advanced Methods for NGS Data Analysis
PART III: Recent Advances in Single-Cell Approaches for Antibody Discovery
- NGS sequencing of single B cell populations
- NGS for analysis of library screening results
- Single-B cell functional analyses via NGS
Instructor Biographies:
Brandon DeKosky, PhD, Assistant Professor, Department of Chemical Engineering, Department of Pharmaceutical Chemistry, Kansas Vaccine Institute, The University of Kansas
Dr. Brandon DeKosky is an Assistant Professor at the University of Kansas Departments of Chemical Engineering and Pharmaceutical Chemistry, where his laboratory leverages recent advances in next-generation DNA sequencing technologies to achieve a
more comprehensive understanding of immune function and accelerate the development of new vaccines and therapeutics. During his graduate research, Dr. DeKosky invented the very first technology for sequencing the antibody proteins encoded by B
cells at the single-cell level, at a massive scale (for example, over 5 million single B cells in a one-day experiment), which reduced cost and enhanced throughput for antibody sequencing by multiple orders of magnitude compared to traditional
antibody discovery platforms. Dr. DeKosky also pioneered the associated bioinformatic methods for rapid statistical analysis of the very large datasets generated by this approach. Ongoing efforts in the DeKosky lab, supported by an NIH Director’s
Early Independence Award and a United States Department of Defense Career Development Award, and focus on expanding our ability to determine the mechanisms of protection for human vaccines and to develop novel and improved targeted human therapeutics.
Matías Gutiérrez
González, PhD, Postdoctoral Researcher, Pharmaceutical Chemistry, The University of Kansas
Dr. Matias Gutierrez is a Postdoctoral Researcher in the lab of Dr. Brandon DeKosky at the University of Kansas. Dr. Gutierrez’s graduate research studied the development and characterization of biopharmaceuticals, with a focus on monoclonal
antibodies. Currently, Dr. Gutierrez works to develop new tools for bioinformatic analysis of single-cell antibody repertoire data, and to establish new techniques for high-throughput B cell sequencing.
WHAT IS A TRAINING SEMINAR?
Each CHI Training Seminar offers 1.5 days of instruction, refreshment breaks, exhibit hall functions, and lunch for all registered attendees on the full day of the class. Each person registered for the Training Seminar will be
provided with a hard copy handbook for the Training Seminar in which they are registered. A limited number of additional handbooks may be available for other delegates. CHI encourages track hopping between conference programs,
and we ask that Training Seminars not be disturbed once they have begun. We ask that attendees commit to attending the entire 1.5-day training, and not engage in track hopping once the class has started.
