DINNER SHORT COURSE: SUNDAY, APRIL 26 (6:00pm-8:00pm)
Sai Reddy, Ph.D., Assistant Professor, Biosystems Science and Engineering, ETH Zurich, Switzerland
Next-generation sequencing (NGS) of antibody repertoires provides a quantitative approach to measuring the diversity and distribution of antibody libraries. This course will enable researchers on how to design, analyze, and perform antibody NGS studies,
which have applications in antibody discovery and engineering. We will go over the practical details of antibody NGS (using the Illumina platform) including library construction and quality control, data processing and analysis, and advanced methods
for improving accuracy by molecular barcoding and error correction.
The course instruction will include:
- Design of antibody sequencing construct for multiplexed library generation
- Quantitative analytical tools for quality control of antibody sequencing libraries
- Preprocessing and quality assessment of antibody NGS data
- Annotation and bioinformatic analysis of antibody NGS data
- The use of unique molecular identifiers (barcodes) for improving accuracy
- Controls for bias and error assessment
Instructor Bio:
Sai Reddy, Ph.D., Assistant Professor, Biosystems Science and Engineering, ETH Zurich, Switzerland
Sai Reddy is a tenure-track Assistant Professor at ETH Zurich in the Dept. of Biosystems Science &
Engineering (since 01.02.2012). His research group focuses on immunogenomics by next-generation sequencing and bioinformatic analysis of immune repertoires and reprogramming of immune cells by genome engineering for applications in biotechnology,
vaccination, and immunotherapy
Prof. Reddy holds B.S. (2003) and M.S. (2004) degrees from Northwestern University (Evanston, IL, USA) in Biomedical Engineering. He completed his Ph.D. thesis at Ecolé Polytechnique Féderale de Lausanne (EPFL, Switzerland) in Bioengineering
and Biotechnology in 2008. Prof. Reddy moved to University of Texas, Austin (USA) for his post-doctoral fellowship (2008-2011), where he worked on protein and antibody engineering.